The spot price rose more than 4% yesterday to US$44.15 per pound, levels last seen in 2012.
Canada-based producer Cameco touched a one-year high intraday before closing flat.
"So uranium is the hot play of the day, but has it run too far and due a breather?" Pepperstone head of research Chris Weston asked, noting uranium plays were getting the attention once reserved for GameStop and AMC.
Gold was steady about $1,790 an ounce on the spot market.
Base metals closed lower in London, although aluminium remains near a 13-year high and the $3,000 per tonne mark.
Chinese output is down amid drives to reduce emissions and conserve power, while a coup in bauxite producer Guinea has raised concerns over the supply of the material used in aluminum production, Bloomberg reported.
Key market futures were up after the US snapped a losing streak yesterday.
Among the mining majors, Rio Tinto rose 0.4% in London, Freeport-McMoRan lost 0.5% in New York and BHP was up slightly in morning Australian trade.